Effect of RNA interference on HIF-2 in the renal cancer cell line 786-0

AIM: To study the effect of RNA interference on hypoxia-inducible factor-2(HIF-2) in the renal cell cancer in vitro and in vivo.METHODS: HIF-2 RNAi was synthesized and inserted into RNA interference eukaryotic expression vector which was confirmed by sequencing.The vector was transfected into the renal cancer cell 786-0 and positive clone was selected by using G418.The HIF-2 expression was detected by RT-PCR and Western blotting method.The growth of cells was measured by MTT method.Nude mouse xenograft assays were also done.RESULTS: Compared with empty vector group and control group,the amounts of HIF-2 mRNA and protein expression were lower in the HIF-2 RNAi group,the difference was significant(P0.01).No significant difference between empty vector group and control group was observed.The cell growth in the HIF2 RNAi group become slower.Compared with control group,the growth of tumor was slower in the RNAi group in the nude mice(P0.01).CONCLUSION: HIF-2 RNAi inhibits the expression of 786-0 and cell growth,and slows the growth of tumor in the nude mice.The result provides new application for biological therapy in the renal cell cancer.