Study on the culture of vibrissa follicles of C57BL/6J mice by the Products of Zhangguang 101 in vitro

Objective To investigate the effects of products of Zhangguang 101 on hair follicles growth in vitro.Methods:Put the separated vibrissa follicles in Williams medium E,the hair follicles were divided into 4 groups:control group,Hair Follicle Nourishing Tonic,Hair Shedding Proof Tonic and Hair Tonic.The change,length and apoptosis cells of the hair follicles were observed.Results:On eight day of culture,the length of hair follicle of control group,Hair Follicle Nourishing Tonic,Hair Shedding Proof Tonic and Hair Tonic were(0.669±0.32)mm,(1.312±0.47)mm,(1.345±0.51)mm and(1.285±0.38)mm respectively compared with control group,the growing speed of hair follicle of three experimental groups were faster,but there is no significant difference among three experimental groups.The results showed that hair bulbs of control group were bulk smaller,texture of hair bulb became loose and hair follicles appeared anaplasia.The results showed that the hair bulbs of experimental groups were circle,its texture was compact,and hair follicles continuously occupied growth period as well as.Apoptosis cells were detected in the hair follicles by use of TUNEL method.It can seen that the number of apoptosis cells per hair follicle in control group,Hair Follicle Nourishing Tonic,Hair Shedding Proof Tonic and Hair Tonic were 21.92±2.11,6.58±1.08,5.83±1.12 and 6.92±1.24 respectively.Compared with three experimental groups,the number of apoptosis cells of control group were more(P0.05),but there is no significant difference among three experimental groups.Conclusions:The products of Zhangguang 101 can promote the growth of vibrissa follicles and delay hair follicle from growth period to catagen.