Study of the cytokines variation during ARDS with experimental model by dexamethasone intervened

Objective: To observe the effects of dexamethasone intervened at different time on TNF-α、IL-8、IL-10 in serum of rabbits with ARDS induced by oleic acid, and to explore the theoretical foundation of glucocorticoids treatmented ARDS. Methods: To induce rabbits' ARDS model through injected oleic acid to rabbits'veins and using radioimmunoassay and enzyme linked immuno sorbent assay(ELISA)to measure the levels of TNF-α、IL-8、IL-10 in serum. Results: In ARDS group the level of TNF-αin serum were significantly higher than those of control group (P0.01);IL-8 in serum were not obvious variation (P 0. 05) ; IL-10 in serum were significantly lower than those in control group (P 0. 01). There were different variations with TNF-α、IL-8、IL-10 in serum when dexamethasone was given at different times. Conclusion: TNF-α、IL-8、L-10 may play important role in the inflammatory process of oleic acid ARDS in rabbits, dexamethasone may relieve lung injuries by intervene discharging of TNF-α、IL-8、IL-10. At the same time, there were possible relation at the progress of inflammation with the intervene time of dexamethasone.