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Impact of compound phyllanthus urinaria(CPUII) on proliferation of HSC-T6 and expression of TIMP-1 mRNA

Liu Ni

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Abstract

Objective To observe the impact of compound phyllanthus urinaria(CPUII) on the proliferation and expression of TIMP-1 mRNA of cultured hepatic stellate cells(HSC-T6),and explore the antifibrotic mechanisms of CPUⅡ Methods HSC-T6 were cultured in vitro and divided into 4 groups randomly,including control group,CPUⅡ5mg/ml group,CPUⅡ2.5mg/ml group,CPUⅡ1.25mg/ml group.After incubation with CPUⅡ,MTT colormetric assay was used to evaluate the inhibitive effect of CPUII on HSC-T6,and the expression of the TIMP-1 mRNA were determined by using semi-quanititative RT-PCR method.Results The levels of proliferation of HSC-T6 and expression of TIMP-1 mRNA in every treatment groups were significantly lower than that of control group.Conclusion CPUII is capable of inhibiting HSC-T6 proliferation and down-regulating the expression of TIMP-1,thus the antifibrotic mechanism of CPUⅡ might be partly due to its downregulation of the expression of TIMP-1 mRNA and acceleration of the degration of ECM in HSC-T6 cells.

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Objective To observe the impact of compound phyllanthus urinaria(CPUII) on the proliferation and expression of TIMP-1 mRNA of cultured hepatic stellate cells(HSC-T6),and explore the antifibrotic mechanisms of CPUⅡ Methods HSC-T6 were cultured in vitro and divided into 4 groups randomly,including control group,CPUⅡ5mg/ml group,CPUⅡ2.5mg/ml group,CPUⅡ1.25mg/ml group.After incubation with CPUⅡ,MTT colormetric assay was used to evaluate the inhibitive effect of CPUII on HSC-T6,and the expression of the TIMP-1 mRNA were determined by using semi-quanititative RT-PCR method.Results The levels of proliferation of HSC-T6 and expression of TIMP-1 mRNA in every treatment groups were significantly lower than that of control group.Conclusion CPUII is capable of inhibiting HSC-T6 proliferation and down-regulating the expression of TIMP-1,thus the antifibrotic mechanism of CPUⅡ might be partly due to its downregulation of the expression of TIMP-1 mRNA and acceleration of the degration of ECM in HSC-T6 cells.

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Available abstract

Objective To observe the impact of compound phyllanthus urinaria(CPUII) on the proliferation and expression of TIMP-1 mRNA of cultured hepatic stellate cells(HSC-T6),and explore the antifibrotic mechanisms of CPUⅡ Methods HSC-T6 were cultured in vitro and divided into 4 groups randomly,including control group,CPUⅡ5mg/ml group,CPUⅡ2.5mg/ml group,CPUⅡ1.25mg/ml group.After incubation with CPUⅡ,MTT colormetric assay was used to evaluate the inhibitive effect of CPUII on HSC-T6,and the expression of the TIMP-1 mRNA were determined by using semi-quanititative RT-PCR method.Results The levels of proliferation of HSC-T6 and expression of TIMP-1 mRNA in every treatment groups were significantly lower than that of control group.Conclusion CPUII is capable of inhibiting HSC-T6 proliferation and down-regulating the expression of TIMP-1,thus the antifibrotic mechanism of CPUⅡ might be partly due to its downregulation of the expression of TIMP-1 mRNA and acceleration of the degration of ECM in HSC-T6 cells.

Key concepts: Downregulation and upregulation, Messenger RNA, Hepatic stellate cell, Chemistry, In vitro, MTT assay, Molecular biology, Andrology

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Impact of compound phyllanthus urinaria(CPUII) on proliferation of HSC-T6 and expression of TIMP-1 mRNA — Research Paper | ScholarLens