An Analysis of Albumin and Globulin of Qinghai Faba Bean Varieties with SDS-PAGE

The albumin and globulin of 2005-00 and Qinghai 13,two varieties of Qinghai Faba bean were studied by improved SDS-PAGE.At the same time by using micro-Kjeldahl Method the appropriate protein content were measured.The result showed that the content of globulin was twice that of albumin.Therefore globulin was the main component of the total protein content.Almost all the albumin and globulin were extracted after extraction for five times with extract solution Ι which included NaCl.But only the globulin were obtained with extract solution Ι for one time after with extraction for four times extract solution Ⅱ which didn't include NaCl,and the globulin had the same mobility as the albumin.The result indicated that albumin was also extracted while extracting globulin,but globulin was not extracted while extracting albumin.