The cultivation of the human umbilical vein endothelial cell in vitro

Objective To explore the culture method of the human umbilical vein endothelial cell(HUVEC) in vitro.Methods HUVECs were acquired with type I collagenase treatment and cultivated in RPMI-1640 containing 20% FBS;the passage was developed with 0.25% trypsinase and 0.02% EDTA.The ECs were identified under the light microscope with immunohistochemical method.Results The primary cells began to attach and grow after being inoculated 4 hours,and grew to monolayer 5-7 days later.They presented cobblestone-appearance with contact inhibition under the light microscope,and the antigens of human Ⅷ factor were positive according to immunohistochemical method.Conclusion Collagenase typeⅠ perfusion is an effective way to cultivate the human umbilical vein endothelial cells in vitro.