Immunogenicity of Human Avian Influenza Split Vaccine to Mice

Objective To observe the immunogenicity of human avian influenza split vaccine to mice.Methods The aluminum adjuvant-containing and adjuvant-free human avian influenza split vaccines with various hemagglutinin contents(20,30 and 45 μg / ml),three batches for each,were used for immunization of mice.Mice were divided various groups,10 for each,and were injected i.p.with 1,2 and 3 doses of the two kinds of vaccines respectively,0.5 ml for each,using those unimmunized as blank control.The interval between doses was one week.Serum samples were collected 21 d after the first dose and determined for HI and neutralizing antibody titers.Results The HI and neutralizing antibody titers of mice immunized with 1 and 2 doses of adjuvant-free vaccines were less than 1 ∶ 40 and less than 1 ∶ 200 respectively.However,the HI antibody titers of mice immunized with 2 and 3 doses of aluminum adjuvant-containing vaccine were more than 1 ∶ 50,and nuetralizing antibody titers with 2 doses of aluminum adjuvant-containing vaccine with hemagglutinin contents of 20 and 30 μg / ml were more than 1 ∶ 200.Neither HI nor neutralizing antibody titers showed significant difference in the mice immunized with 2 and 3 doses of aluminum-containing vaccines with hemagglutinin contents of 20,30 and 45 μg / ml(P 0.05).However,both HI and neutralizing antibody titers of mice immunized with 2 and 3 doses of aluminum adjuvant-containing vaccine of three batches were significantly higher than those with adjuvant-free vaccine of the corresponding batches.Conclusion The immunogenicity of aluminum-containing vaccine was significantly superior to that of adjuvant-free vaccine,and the immune effects of 2 and 3 doses of aluminum-containing vaccine with hemagglutinin contents of 20 and 30 μg / ml were satisfactory.