Effect of Reactive Oxygen Species on Activity of Antioxidant Enzymes in Leaf Disks of Vitis vulpine

Effect of reactive oxygen species on the activity of antioxidant enzymes in leaf disks of Vitis vulpine was investigated. The data revealed that cell membrane permeability of the leaf disks was obviously enhanced by in vitro treatment with hydrogen peroxide and hydrogen peroxide plus Fe2+. Activity of ascorbate peroxidase, guaiacol peroxidase, and superoxide dismutase was significantly reduced by the treatment of reactive oxygen species, but H2O2 with low concentrations could enhance catalase activity to certain degree. The cell membrane permeability was related to the concentration of reactive oxygen species and positively significantly.