The cloning of 26kDa outer membrane protein gene of Helicobacter pylori

Objective: To construct a recombinant vector which could express 26kDa outer membrane protein(OMP) from Helicobacter pylori(Hp) ,and exploit the possibility obtaining the vaccine conferring protection from Hp infection and a diagnostic reagent kit quickly detecting Hp infection. Methods: The gene encoding the structural 26kDa OMP of Hp was amplified from Hp chromosomal DNA by PCR, and inserted in the prokaryotic expression vector pET32a(+), and transformed into the Top10 E. coli strain. Recombinant vector was selected and identified to be transformed into BL-21(DE3) E. coli strain and expressed recombinant protein. Results: The cloned gene of 26kDa OMP was amplified to be 594 base pairs(bp), 1.1% of the cloned gene was mutated , 1.51% of amino acid radicles was changed. Compared with reports: there was high homogenicity between them. Conclusion: This result suggested that recombinant vector expressing 26kDa OMP may be an potential source obtaining the vaccine conferring protection from Hp infection and a diagnostic reagent kit quickly detecting Hp infection.