Effects of Shenfu injection on the permeability of blood brain barrier and the nervous function of focal cerebral ischemic reperfusion injured rats

Objective:To investigate the effects of Shenfu injection(SFI)on the permeability of blood brain barrier(BBB)and the nervous function of focal cerebral ischemic-reperfusion injured rats,and judge if SFI has protective effects for focal cerebral ischemic-reperfusion injured rats to provide theoretical proof for the clinical application of SFI.Methods:Eighty male SD rats were divided into four groups randomly:group A(the sham operated control group),group B(the sham operated trial group),group C(the control group)and group D(the trial group).The rats in group A and group C received i.v.a bolus of normal saline(NS)for 10 mg/kg 5 min after anesthesia,while those in group B and group D received a bolus of SFI for 10 mg/kg instead.The focal cerebral ischemic-reperfusion injury model was established with thread embolism of middle cerebral artery(MCA)according to Zea Longa’s method in group C and group D.The time for postanesthetic revival in each group was recorded.The Longa score in each group was recorded at 2 h of occlusion and 24 h of reperfusion.The injury to the blood-brain barrier was observed by the content of Evan’s blue(EB)in the cerebral tissue,the changes of the structure of BBB were observed with electron microscope at the end of the experiment.Results:The body weight in each group had no statistical difference.The time for revival from anesthesia in group A and group B was shorter than that in group C and group D(P0.05),and that in group C was longer than that in group D(P 0.05)but there was no difference between group A and group B.After ischemia for two hours,Longa score in group A and group B was lower than that in group C and group D(P0.01),and there was no difference either between group A and group B,group C and group D.After reperfusion for 24 h,Longa score in group A and group B was lower than thatin group C and group D,there was no difference between group A and group B(P0.01),and Longa score in group C was higher than that in group D(P0.01).At the 24th hour of reperfusion,Longa score in group C was higher than that at the 2nd hour of ischemia(P0.01),but there was no difference for group D.After ischemia for 2 h and reperfusion for 24 h,the rats in group A and group B had an evidently lower Evan’s blue content in the cerebral tissue than that of the rats in group C and group D,and there was no differe ncebetween group A and group B,while that of the rats in group C was higher than that of the rats in group D.Under the electron microscope,brain edema and microvessel pressed in group C were more severe compared with those in group D.But there was no significant abnormality in group A and group B.Conclusion:Shenfu injection could reduce blood-brain barrier permeability after focal cerebral is-chemic-reperfusion injury and it has significant nervous function protection against ischemic-reperfusion damage.