2008•Zhongguo sheng-hua yaowu zazhiRequires access
Identification and immunogenicity analysis of artificial antigen of Glycyrrhizic acid conjugated with BSA
XU Jin-sen
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Abstract
Purpose To prepare the conjugate antigen Glycyrrhizic acid-bovine serum albumin(GA-BSA) and its antiserum to produce anti-GA monoclonal antibody and to establish a fast assay for GA content by an ELISA.Methods The conjugate antigen GA-BSA was artificially coupled and identified by matrix-assisted laser absorption ionization time-of-flight mass spectrometry(MALDI-TOF-MS).BALB/c mice were immunized with GA-BSA to prepare antiserum against GA.The antiserum titer and specificity were detected by indirect ELISA and competitive ELISA,respectively.Results The conjugating ratio of GA and carrier BSA was 7∶1.The antiserum titer was 1∶8 000 and it reacted specifically to the GA.Conclusion The successfully synthesized conjugate antigen GA-BSA implies its feasibility in the establishment of fast immunoassay for the GA content determination