Construction of Eukaryotic Plasmid Expressing Murine Interleukin-2(mIL-2) and Analysis of Expressed Product

Objective To construct an eukaryotic expression system for recombinant murine interleukin-2(mIL-2) and use the recombinant plasmid as an adjuvant to enhance the immunogenicity of DNA vaccine.Methods Extract total RNA from murine splenocytes immunized with PMA and amplify mIL-2 gene by RT-PCR.Clone the amplified gene into an eukaryotic expression vector VR1012 and transfect to COS-7 cells.Split the transfected cells and identify the expressed product by Western blot.Results A mIL-2 gene sequence with signal peptide was obtained and inserted into plasmid VR1012 correctly.Western blot proved that mIL-2 was expressed.Conclusion An eukaryotic expression system for mIL-2 was constructed,and mIL-2 with biological activity was obtained.It laid a foundation of large-scale production of mIL-2 protein.