2004•Chinese Journal of Aesthetic MedicineRequires access
The modulation of Smad3 mRNA and Smad7 mRNA expression in keloid fibroblasts
Han Yan
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Abstract
Objective To study the modulation of Smad3 mRNA and Smad7 mRNA expression in keloid fibroblasts(KFB) by transforming growth factor-β1(TGF-β1). Methods The de novo protein sythesis and mRNA in KFB were inhibited by pretreatments with cycloheximide(CHX) and actinomycin D.The levels of Smad3 mRNA and Smad7 mRNA expression in KFB were detected by using method of RT-PCR. Results Inhibition of de novo protein synthesis in KFB caused a modest increase in Smad3 mRNA levels;however,delayed down-regulation by TGF-β1 was completely prevented(P0.01).In contrast,induction of Smad7 mRNA in these cells was unaffected by pretreatment with CHX.The levels of Smad3 mRNA with less differences in untreated and TGF-β1-treated KFB. Conclusion The modulation of Smad3 mRNA expression by TGF-β1 was required de novo protein synthesis,while rapid and transient stimulation of Smad7 mRNA did not require it,suggesting that the Smad7 gene is a direct target of receptor-activated SMAD signals in KFB.