Effect of hydrogen sulfide on myocardial hypoxia/reoxygenation injury in vivo

AIM:To investigate the changes of endogenous cystathionine-γ-lyase/hydrogen sulfide pathway on hypoxia/reoxygenation injury in vivo and to explore the relationship between this pathway and hypoxia/reoxygenation injury.METHODS:Primary myocardial cell culture in vivo came from Wistar baby rats born less than 48 h.The cells were under the conditions of hypoxia(2% O2,5% CO2) for 3 h and reoxygenation(21% O2,5% CO2) for 2 h to induce hypoxia/reoxygenation injury.MTT was used to detect the cell survival in every group.The activities of lactate dehydrogenase(LDH) in culture medium,malondialdehyde(MDA) and superoxide dismutase(SOD) in myocardial cells were measured with colorimetry method.RT-PCR method was used to test CSE mRNA expression in myocardial cells.RESULTS:Compared to IR group,the cells in NaHS+IR and IR+NaHS groups had a higher survival rate,lower LDH concentration in culture medium,higher SOD activity and lower MDA in myocardial cells.At the same time,the results of RT-PCR displayed that CSE mRNA were down-regulated in myocardial cells after hypoxia/reoxygenation injury,and if CSE inhibitor PPG was added into the culture medium before hypoxia,no protective effects were detected.CONCLUSION:NaHS might protect the myocardial cells from hypoxia/reoxygenation injury through decreasing oxygen free radical production and stabilizing the cell membrane.