A study on the effect of NGF,CNTF and GDNF applied in combination on the structure and function recovery of sciatic nerve of rats

Objective: To investigate the effect of NGF,CNTF and GDNF in combination on the structure and function recovery of sciatic nerve of rats.Method:The model of sciatic nerve gap defect of rats was used. The sciatic nerve function index(SFI),nerve electrophysiological parameter(nerve conduction velocity,NCV;compound muscle action potential,CMAP),recovery rate of gastrocnemius muscle wet weight and the morphological parameter of regenerated nerve fiber were measured under various kinds of treatment of NGF,CNTF,GDNF,NGF+CNTF,CNTF+GDNF,NGF+GDNF,NGF+CNTF+GDNF and in control group respectively.Result: In the group of NGF,CNTF and GDNF applied in combination, SFI,NCV,CMAP,and recovery rate of gastrocnemius muscle wet weight were better than those in all other groups; the average diameter of nerve fiber, amount of regenerated axon and area of nerve tissue were greater than those in other groups, except the thickness of myelin sheath.Conclusion:The effects of NGF,CNTF and GDNF applied in combination on the structure and function recovery of regenerated nerve were better than single or two neurotrophic factors.