Antiviral effect of arbidol hydrochloride against respiratory syncytial virus in vitro

Objective To study the antiviral effect of arbidol hydrochloride(Ahyd) on respiratory syncytial virus in vitro. Methods Multi-mode administration and multi-dose were used to investigate the mechanism of the antiviral activi-ty. Cell survival rate of each testing group infected by the virus was measured using 3-4,5-dimethylthiazo(-2-yl)-2,5,di-phenyle-tetrazolium bromide(MTT) assay,and reverse transcription PCR(RT-PCR) was applied to detect virus amount of each testing group. Results Survival rate of viral group reached to 20. 2%,and that of Ahyd treated group was up to 71. 8% with a linear dependent manner in the range of no-toxicity dose. Another important results showed that 2-△△Ct of viral group was 9. 33 and that of Ahyd treated group was decreased to 3. 21,which was equal to one third of that of vi-ral group. Conclusion Ahyd has a significant preventive and therapeutic effect on the infection caused by respiratory syncytial virus.