Effects of Icarrin on Osterix in Rat Osteoblast

Objective To observe the effects of icarrin on osteoblast activity and to investigate the effects of icarrin on Osterix in osteoblast.Methods The culture medium with different icarrin concentrations (1 ng/ml,10 ng/ml,100 ng/ml) and the second generation osteoblasts from the skull of newborn SD rats were mixed for the observation respectively on the proliferation and the differentiation of the OB. Osterix mRNA expressions were quantified by RT-PCR.Results In comparison with the control group,the OD values increased in a concentration-dependent manner(P0.01 significant, Icarrin at 100 ng/ml);the ALP activity increased too(significant,≥10 ng/ml). Icarrin at 1 ng/ml,10 ng/ml,100 ng/ml increased Osx mRNA expressions(P0.05),with the maximal effect at the concentration at 10 ng/ml.Conclusion The icarrin had the effects on stimulating the proliferation and maturation of cultured osteoblast in vitro and could increase Osx mRNA expressions.