Influence of survivin gene RNA interference on the sensitivity to paclitaxel in breast carcinoma cell Line MCF-7

Objective To investigate the effect of Survivin gane RNA interference on the sensitiv- ity of breast cancer cell line MCF-7 to paclitaxel.Methods MCF-7 calls were transfected with recom- bined eukaryotic expression plasmid pSurvivin shRNA by Lipofectamine 2000,and then the expressions of Survivin were detected at mRNA and protein level.After incubation with Paclitaxel,proliferation inhibition rates and apoptosis rates were analyzed by tetrazolium bromide(MTr)colorimetry and Annexin-V stai- ning.The changes of Survivin expression were detected by Western blot analysis.Results Compared with cells transfected with negative control plasmid and untransfected cells,the expressions of Survivin in the cells transfeoted with pSurvivin shRNA declined significantly at mRNA and protein levels.Proliferation in- hibition rate and cell apoptosis rate were higher in cells transfected with pSurvivin shRNA when treated with Paclitaxel.Moreover,in this process the expression of survivin in cells trandected with pSurvivin shR- NA did not increase at early time(within 6 hours),while untansfected cells showed an obvious increase within 6 hours.Conclusions Targeted inhibition of survivin by shRNA method could enhance the sensi- tivity of cancer cells to paclitaxel.