2008•Unpublished venueRequires access
Study on the effects of Sapylin on human gastric cancer cell SGC7901 in vitro
Min Ye
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Abstract
Objective:To study the effects of Sapylin on human gastric cancer cell SGC7901 in vitro.Methods: SGC7901 cells were treated with 0,0.001,0.01,0.1 and 0.5 KE/ml Sapylin for 6,12,24,48 hours respectively.The cell growth condition was observed by MTT assey;The cell cycle was analyzed by flow cytometry;Immunocytochemical staining was used to analyze the expression of p53,p27 and PCNA protein in SGC7901 cell between control group and treated group.Results: Sapyin inhibited the proliferation of SGC7901 cells by dose-dependent and time-dependent manner.SGC7901 cells were treated with 0.5 KE/ml Sapylin for 48 hours,cells in G0/G1-phase were decreased in cell cycle,but increased in S-phase(P0.01).Results of immunocytochemical staining showed that Sapylin can down-regulated the expression of p53 protein,up-regulated the p27 protein(P0.01).The expression of PCNA showed no significant difference between control group and treated group(P0.01).Conclusion:Sapylin can change the cell cycle,inhibit the proliferation of human gastric cancer SGC7901cell.It can also increase the expression of p53 protein,but decrease the p27 protein.