Genetic Polymorphism of Mitochondrial DNA in Ethnic Miao,Buyi and Dong from Guizhou

Objective: To study the population genetics characteristics of mitochondrial DNA in ethnic Miao,Buyi and Dong populations from Guihzou province. Method: Polymerase chain reaction and restriciton fragment length polymorphism( PCR- RFLP) was used to detect the 12 SNP loci in a total of 285 samples. The result was verified with DNA sequencing. Result: The 47 haplotypes and 12 haplogroups were detected in 285 samples. The main haplotypes were H32,H36 and H10( 41. 04%),but the difference was statistically significant among the three ethnic groups( P 0. 05). There were 11 haplotypes were found in all the groups. By the way,H11 was only found in Miao population( 11. 58%). Haplogroup B and M7 were the predominant types( both of 21. 05%). In haplogroup B,Miao and Buyi had difference with Dong respectively and the difference was statistically significant( P 0. 05). Conclusion: There was greatly similarity in the distribution of the haplotype of the mtDNA among the three ethnic groups,but there were some difference in the distribution of haplotypes. Haplogroups and Principal Component( PC) analysis showed that the genetic polymorphism of mtDNA of the three populations indicated their origination from southern China. The matrilineal genetic structure of Dong population is more complex than the others,but they all have the possibility of gene flow.