Patterns of notch-1 expression in papillary thyroid cancer and their oncological significance

Objective:To study the Notch-1 mRNA and its protein expression in papillary thyroid cancer and analyze its clinical biological significance.Methods:Using RT-PCR technology and immunofluorescence in exactly the same experimental conditions,were used to detect human thyroid papillary carcinoma tissues,compare with normal thyroid tissues.Results: RT-PCR in thyroid papillary cancer tissues,the expression of Notch-1 mRNA were significantly weaker than normal thyroid tissue(P0.05);In immunofluorescence,Notch-1 in thyroid carcinoma and normal thyroid tissue were expressed,but in thyroid cancer tissue is more weaker expressed than in normal thyroid tissue.Notch-1 gene expression in papillary thyroid carcinoma and normal thyroid tissue was statistically significant difference between(P0.001).Conclusion:Notch signaling pathway in thyroid cancer tissues showed steady-state inactivation,and thyroid cancer occurrence and development are closely linked.Notch-1 in papillary thyroid carcinomas with low expression,it may be the process in the thyroid cancer is a tumor suppressor gene.