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2002•Acta Academiae Medicinae JiangxiRequires access

Effect of Up-expression of Fas Ligand on the Apoptosis of Gastric Carcinoma

Qun Huang

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Abstract

Objective:To investigate the effect of up-regulation of FasL ligand protein on the apoptosis of gastric carcinoma(GC). Methods: expression of FasL protein in 50 resected GC and apoptosis of cancer cells were detected with immunohistochemistry and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL). Results: The expressions of FasL were detected in all of 50 resected GC with positive rate of 100%, which was significantly higher than that (45%) in control normal gastric epithelia( P 0.01).In contrast, the FasL expression was mediated or extensive in 78% of GC,( P 0.01).As the FasL expression became more extensive, the apoptosis index of GC cells decreased stepwisedly. Conclusion:Human GC may down regulate its apoptosis through up regulating its expression of FasL.Therefore,the cancer may protect itself from immune surveillence.

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Objective:To investigate the effect of up-regulation of FasL ligand protein on the apoptosis of gastric carcinoma(GC). Methods: expression of FasL protein in 50 resected GC and apoptosis of cancer cells were detected with immunohistochemistry and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL). Results: The expressions of FasL were detected in all of 50 resected GC with positive rate of 100%, which was significantly higher than that (45%) in control normal gastric epithelia( P 0.01).In contrast, the FasL expression was mediated or extensive in 78% of GC,( P 0.01).As the FasL expression became more extensive, the apoptosis index of GC cells decreased stepwisedly. Conclusion:Human GC may down regulate its apoptosis through up regulating its expression of FasL.Therefore,the cancer may protect itself from immune surveillence.

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Available abstract

Objective:To investigate the effect of up-regulation of FasL ligand protein on the apoptosis of gastric carcinoma(GC). Methods: expression of FasL protein in 50 resected GC and apoptosis of cancer cells were detected with immunohistochemistry and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL). Results: The expressions of FasL were detected in all of 50 resected GC with positive rate of 100%, which was significantly higher than that (45%) in control normal gastric epithelia( P 0.01).In contrast, the FasL expression was mediated or extensive in 78% of GC,( P 0.01).As the FasL expression became more extensive, the apoptosis index of GC cells decreased stepwisedly. Conclusion:Human GC may down regulate its apoptosis through up regulating its expression of FasL.Therefore,the cancer may protect itself from immune surveillence.

Key concepts: Fas ligand, TUNEL assay, Apoptosis, Immunohistochemistry, Terminal deoxynucleotidyl transferase, Cancer research, Cancer, Molecular biology

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Effect of Up-expression of Fas Ligand on the Apoptosis of Gastric Carcinoma — Research Paper | ScholarLens