The effects of dietary chitosan supplementation on the survival,serum biochemical indicators and non-specific immunity of Procambarus clarkii

The present stuty was conducted to investigate the effects of dietary chitosan on survival,blood biochemical indices and non-specific immunity of Procambarus clarkii.Six diets were formulated by adding 0.0%(control group),0.5%,1.0%,1.5%,2.0%,3.0% chitosan in basal diet.P.clarkii with initial body weight of(21.55±1.62) g were fed the six diets for 60 days.Results indicated that:the survival rate of 0.5% chitosan addition group was about 84.44%,there was no significant difference between control group and 1.0% addition group.Total molting rate and relative growth rate increased with the content of chitosan at first,then decreased,total molting rate and relative growth rate in were Significant higher than control group.There was no significant difference of hepatopancreas index in each treatment group.Serum total protein,albumin and total cholesterol of 2.0% chitosan addition group were significantly higher than control group.Serum triglyceride of the five chitosan addition groups were significantly higher than that of control group.Lysozyme(LZM)and superoxide dismutase(SOD)activity in blood serum and hepatopancreas of 1.5% chitosan addition group were significantly higher than control group.Compared to the control group,serum acid phosphatase(ACP) and catalase(CAT)of 0.5% chitosan addition group increased significantly.Hepatopancreas CAT activity of 0.5% addition group was the highest,(4.93 U) and significantly higher than control group.In conclusion,adding chitosan in diet could improve P.clarkii growth performance and non-specific immune function.The addition of chitosan was suggested to be 0.5%~1.5%.