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2013•Chinese Journal of Hospital PharmacyRequires access

Protective effect of mogroside against H_2O_2 induced apoptosis in PC12 cell

Liang Li-yin

Open publisher page 2 citations

Abstract

OBJECTIVE To investigate the protective effect of mogroside on exogenous oxidant induced apoptosis in PC12 cell.METHODS PC12 cells were pre treated with 0.1 and 1 μg·mL-1 mogroside,respectively,followed by 1 h of 400 μM H2O2 incubation.After incubation,cell apoptosis rate was determined by flow cytometry,moreover,Bcl-2 and Bax protein levels were detected by western blot method.RESULTS Apoptosis rate risen by H2O2 was alleviated by pre-incubate with mogroside at 0.1 and 1 μg·mL-1(P0.001).Furthermore,Bcl-2 protein expression was increased,while Bax expression was decreased as well as Bax/Bcl-2 ratio(P0.001).CONCLUSION Mogroside demonstrated protective effect on PC12 cell against H2O2 induced apoptosis,which might be mediated via Bcl-2 family of apoptosis regulatory proteins regulation.

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OBJECTIVE To investigate the protective effect of mogroside on exogenous oxidant induced apoptosis in PC12 cell.METHODS PC12 cells were pre treated with 0.1 and 1 μg·mL-1 mogroside,respectively,followed by 1 h of 400 μM H2O2 incubation.After incubation,cell apoptosis rate was determined by flow cytometry,moreover,Bcl-2 and Bax protein levels were detected by western blot method.RESULTS Apoptosis rate risen by H2O2 was alleviated by pre-incubate with mogroside at 0.1 and 1 μg·mL-1(P0.001).Furthermore,Bcl-2 protein expression was increased,while Bax expression was decreased as well as Bax/Bcl-2 ratio(P0.001).CONCLUSION Mogroside demonstrated protective effect on PC12 cell against H2O2 induced apoptosis,which might be mediated via Bcl-2 family of apoptosis regulatory proteins regulation.

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Available abstract

OBJECTIVE To investigate the protective effect of mogroside on exogenous oxidant induced apoptosis in PC12 cell.METHODS PC12 cells were pre treated with 0.1 and 1 μg·mL-1 mogroside,respectively,followed by 1 h of 400 μM H2O2 incubation.After incubation,cell apoptosis rate was determined by flow cytometry,moreover,Bcl-2 and Bax protein levels were detected by western blot method.RESULTS Apoptosis rate risen by H2O2 was alleviated by pre-incubate with mogroside at 0.1 and 1 μg·mL-1(P0.001).Furthermore,Bcl-2 protein expression was increased,while Bax expression was decreased as well as Bax/Bcl-2 ratio(P0.001).CONCLUSION Mogroside demonstrated protective effect on PC12 cell against H2O2 induced apoptosis,which might be mediated via Bcl-2 family of apoptosis regulatory proteins regulation.

Key concepts: Apoptosis, BAX Protein, Incubation, Chemistry, Flow cytometry, Western blot, Cell, Molecular biology

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