Inhibitory effect of rosiglitazone combined with cisplatin on the growth of endometrial carcinoma in Nude Mice

Objective To investigate the synergistic effects of rosiglitazone(ROZ)and cisplatin on the growth of the human endometrial cancer cell line KLE xenografted to nude mice.Methods KLE cells were transplanted into nude mice to form the study model.When the models were established,they were randomly divided into 6 groups:the control group(A),the cisplatin1 mg/kg group(B),the cisplatin 3 mg/kg group(C),the ROZ 50 mg/kg group(D),the cisplatin1 mg/kg+ROZ 50 mg/kg group(E),and cisplatin 3 mg/kg+ROZ 50 mg/kg group(F).Cisplatin was given by intraperitoneal injection every two days and ROZ was intragastrically administered every day.During the period of observation,the volumes of the transplanted tumors were measured every 4 days.The tumor growth inhibitory rates of each group were calculated.Also,the curves of BALB/c-nude mice growth were drawn.After 38 days,BALB/c-nude mice were euthanized and xenograft tumors were measured.Expression of NF-κB and PTEN protein in subcutaneous tumors were determined by immunohistochemical method with an image analysis system.Results Significant differences in tumor inhibitory rates were found between the treatment groups and the control group(P0.05).The tumor inhibitory rate of B,C,D,E,F 5 group was 24.41%,43.34%,49.67%,78.02%,84.78%,respectively,and the combination group showed synergistic effect on inhibiting the growth of the xenografts.Contrary to the control group,expression of subcutaneous tumor NF-κB was down-regulated and PTEN was obviously up-regulated by rosiglitazone and its combination with cisplatin.Conclusion Rosiglitazone could enhance inhibition of the growth of endometrial tumor in combination with cisplatin.