Inhibition Effect of Eriocalyxin B against Angiogenesis of Hepatocelluar Carcinoma and Human Normal Endothelial Cell

OBJECTIVE:To study the effect of eriocalyxin B (ER-B) on the proliferation of human umbilical vein endothelial cell (HUVEC) and hepatocelluar carcinoma angiogenesis. METHODS:The effects of ER-B at 6 kinds of concentration on the proliferation of HUVEC cultured in vitro were detected using modified MTT assay. Human hepatic cancer chicken chorioallantoic membrane angiogenesis model was induced to determine the inhibition effect of ER-B against angiogenesis. RESULTS:ER-B showed significant inhibitory effects on the proliferation of HUVEC in a dose and time dependent manner with IC50 about 7.27 μg·mL-1. The moderate inhibition rate of ER-B in dose of 25 μg·mL-1,50 μg·mL-1 and 100 μg·mL-1 to area of human hepatic cancer chicken chorioallantoic membrane angiogenesis were 14.15%,48.72% and 60.63%,respectively. 50 μg·mL-1 ER-B has same effect to positive control. CONCLUSION:ER-B significantly inhibits the proliferation of HUVEC and chicken chorioallantoic membrane angiogenesis induced by human liver cancer cell.