Co-expression of bone morphogenetic protein 9-2 induces differentiation of multipotential stem cells C3H10 into osteoblasts

Objective To investigate the capacity of co-expression of bone morphogenetic protein 9-2(BMP9-2) in inducing the differentiation of multipotential stem cells C3H10 into osteoblasts.Methods C3H10 cells were divided into four groups,and infected with recombinant adenoviruses with BMP9-2,BMP2,BMP9 and GFP respectively.The induction of differentiation of C3H10 cells to osteogenesis by co-expression of BMP9-2 was observed by alkaline phosphatase(ALP) staining,alkaline phosphatase quantitation and calcium-alizarin red staining.Results The co-expression of BMP9-2 induced the expression of ALP in C3H10 cells.The positive rate of ALP in BMP9-2 group was 1.6 times higher than that in BMP2 group,2.5 times higher than that in BMP9 group,and 4 times higher than that in GFP group.The activities(A520) of ALP in BMP9-2 group 5,7 and 9 d after infection were higher than those in BMP2,BMP9 and GFP groups.The co-expression of BMP9-2 induced the sedimentation of calcium salt in C3H10 cells.Calcified tuberculum was observed in C3H10 cells 14 d after infection with BMP9-2 by calcium-alizarin red staining.The sedimentation level in BMP9-2 group was 1.4 times higher than that in BMP2 group,1.3 times higher than that in BMP2 group,and 5.2 times higher than that in GFP group.Conclusion The co-expression of BMP9-2 may induce the differentiation of C3H10 cells into osteoblasts,of which the ability in inducing osteogenesis is higher than those of BMP2 and BMP9.