2013•The Chinese Journal of Cardiac Pacing and ElectrophysiologyRequires access
The effection of myocardial tissue engineering patch on ventricular electrical activity and autonomic nerve remodeling in myocardial infarction rat
MA Yi-ton
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Abstract
Objective To explore effection on myocardial tissue engineering patch on ventricular electromyographic activity and autonomic neural remodeling in myocardial infarction(MI) SD rat by electrophysiological techniques and immunohistochemical technique.Methods Forty-five SD rats of either sex weighting 220-250 g were euthanized under chlorine hydrate 0.3 ml/100 mg anesthesia and were randomly divided into three groups: sham operation group(n=15),MI group(n=15) and transplantation group(n=15).Sham operation group only open chest,without coronary artery ligation, while the MI group were ligated the coronary artery.The rats after 1 weeks of MI model establishment were chest opened again with left ventricular wall of the heart exposured,and the myocardial tissue engineering layers were sutured to the four corners of infarct area with NO1 silk in transplantation group,which were closely contacted with the infarction area.Myocardial tissue were examined by light microscopy with HE staining,GAP43,TH and ChAT expression of immunoreactive protein were examined with immunohistochemical technique.Results ①Compared with the the sham operation group,ERP of infarcted border zone was significantly shortened in MI group(P0.01);and ERP of infarcted border zone was prolonged in transplantation group(P0.01).②Immunohistochemistry of left ventricular GAP43,TH and ChAT could be seen under the light microscope in three groups rats,Compared with the sham operation group,GAP43 had a significantly higher expression in transplantation group(P0.01).TH and ChAT had no significantly higher expression in transplantation group(P 0.01),respectively. Compared with the sham operation group,GAP43,TH and ChAT had significantly higher expression in MI group (P0.01),respectively.Conclusion Myocardial tissue engineering patch has a contractive,electrophysiological stability,which can improve integration recovery of heart with damaged myocardial electrophysiological properties and autonomic nerve regeneration and repair.