Effect of hydrogen sulfide on acute lung injury induced by lipopolysaccharide in rats

Aim To study the effect of hydrogen sulfide(H2S)on acute lung injury induced by lipopolysaccharide(LPS) in rats.Methods Sixty-four ♂ rats were randomly divided into eight groups(n=8).Ⅰ control(3 h) group;ⅡLPS 3 h group;Ⅲ control(6 h) group;Ⅳ LPS 6 h group;Ⅴ LPS+NaHS low dose group;Ⅵ LPS+NaHS middle dose group;Ⅶ LPS+NaHS high dose group;Ⅷ LPS+PPG group.In LPS 3 h and LPS 6h groups,LPS was administrated.Saline was administrated in control groups.The rats were killed at 3 h or 6 h respectively after administration of LPS.In LPS+NaHS low,middle,high dose groups or LPS+PPG group,sodium hydrosulfide(NaHS) or DL-propargylglycine(PPG) were administrated at 3 h after administration of LPS respectively.The rats were killed at 3 h after administration of NaHS or PPG.The morphological changes of lung tissues were observed by light and electron microscopes respectively.The lung coefficient and wet to dry lung tissues weight(W/D) were respectively measured.The content of H2S in plasma and activity of cystathionine-γ-lyase(CSE) in lung tissues were respectively detected.The content of malondialdehyde(MDA),and activities of superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) in lung tissues were respectively measured.Results In the LPS 3 h and LPS 6 h groups,the lung tissues were significantly injuried.The lung coefficient,W/D and content of MDA were signifcantly increased compared with those of the control group.The content of H2S in plasma,activities of CSE,SOD and GSH-Px in lung tissues were respectively decreased compared with those of the control group.Compared with the LPS 6 h group the injury of the lung tissues was markedly ameliorated,the coefficient,wet-to-dry weight ratio and content of MDA in lung tissues were respectively decreased and the content of H2S in plasma and activities of CSE,SOD and GSH-Px in lung tissues were significantly increased in the LPS+NaHS low,middle and high dose groups.Compared with the LPS 6 h group the injury was aggravated,the coefficient,wet-to-dry weight ratio and content of MDA in lung tissues were increased,the content of H2S in plasma and activities of CSE and SOD in lung tissues were decreased in the LPS+PPG group,but activity of GSH-Px was not markedly altered.Conclusion The CSE/H2S system might be play a role in the physiopathologic process of the ALI induced by LPS in rats.The administration of NaHS could ameliorate the ALI.