Effect of recombinant adenovirus livin siRNA on proliferation and apoptosis of U251 cells

Objective To investigate the silencing effect of siRNA targeting livin gene on the proliferation and apoptosis of U251 cells.Methods Adenoviral vector of siRNA targeting livin was constructed and transfected into U251 cells.The gene silencing efficiency was monitored by RT-PCR and Western blotting analysis;the proliferation and apoptosis of U251 cells were detected by MTT assays and flow cytometry,respectively.Meanwhile,the therapeutic effect of the recombinant adenovirus was also observed in tumor-bearing mice.Results The constructed recombinant adenovirus effectively inhibited the expression of livin at both mRNA and protein levels;it also inhibited the proliferation and promoted the apoptosis of U251 cells.In addition,the recombinant adenovirus effectively inhibited the tumor growth in mice and improved the mean survival period of tumor-bearing mice.Conclusion Silencing livin gene expression with recombination adenovirus carrying siRNA targeting livin can inhibit the proliferation and promote the apoptosis of U251 cells,which casts new lights on the treatment of glioma.