Effects of oleanic acid on apoptosis of PC12 cells induced by Aβ25-35

【Objective】 To investigate protective effects of oleanic acid on apoptosis of PC12 cells induced by β-amyloidprotein(Aβ25-35).【Methods】 The PC12 cells were divided into control group,model group induced by Aβ25-35,OA groups(OA at 80,40,20 μg/mL were added into the cultures of PC12 cells in the presence of 20 μmol/L Aβ25-35).Cell viability was detected by CKK-8 method.The content of LDH was determined by Microplate Reader method.The apoptotic rates were examined by Annexin V-FITC and staining with PI.The mRNA expression of bcl-2 and bax was quantified by real-time PCR.【Results】 Cells were injuried by Aβ25-35 in model group.Compared with the model group,cell viability was improved,the content of LDH in supernatant fluid was reduced,and the apoptotic rates was reduced in OA groups(P 0.05).In addition,mRNA expression of bcl-2 increased and that of bax decreased(P 0.05),then between 20 μg/mL OA group and model group bcl-2 transcription level was not different.【Conclusion】 OA inhibits apoptosis of PC12 cells induced by Aβ25-35,which may be involved adjust Bcl-2 and Bax gene expression in the mechanism of anti-apoptosis.