The Effect of Gui Fu Di Huang Bolus on the Marrow Derived Cells of Senile Dog

Aim To observe the effect of a Chinese traditional medicine - Gui Fu Di Huang bolus on the cellular proliferation of the senile dog bone marrow mesenchymal stem cells (MSCs). Methods Four senile hybrid dogs were involved in this experiment and the boluses were given orally 1 pill a day for 20 days to the dog. Before and after the boluses applied, bone marrow mesenchymal mononuclear cells were extracted, isolated and cultured at the density of 1×10\+5/ml, 1×10\+6/ml, 1×10\+7/ml in 24 well plates. Osteogenic differentiation test and adipogenic differentation test were performed to detect the multilineage differentiation ability of the obtained cells. Results: After the treatment, the cells could grow and reexpand whereas the cells obtained before the boluses applied were difficult to grow. The cells of post-treatment cultured for multilineage differentation is positive to oil red-O staining, and positive to alizarin red staining, Von Kossa staining and Alkaline phosphatase staining after osteogenic differentiation. Conclusion Gui Fu Di Huang bolus can promote the proliferation of the senile dog bone marrow mesenchymal stem cells(MSCs).