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A Simple Method of Protein Staining with CBB G250 .

Kaili Wu

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Abstract

A simple method of protein staining with Coomassie Brilliant Blue G250 is introduced, which had almost no background,needed less time and only two reagents. The working concentration of Coomassie Brilliant Blue G250 in the staining system with diluted hydrochloric acid was 0 0015%, and the sensitivity was 0 02 μg protein per electrophoresis zone.

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What this paper is about

A simple method of protein staining with Coomassie Brilliant Blue G250 is introduced, which had almost no background,needed less time and only two reagents. The working concentration of Coomassie Brilliant Blue G250 in the staining system with diluted hydrochloric acid was 0 0015%, and the sensitivity was 0 02 μg protein per electrophoresis zone.

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Available abstract

A simple method of protein staining with Coomassie Brilliant Blue G250 is introduced, which had almost no background,needed less time and only two reagents. The working concentration of Coomassie Brilliant Blue G250 in the staining system with diluted hydrochloric acid was 0 0015%, and the sensitivity was 0 02 μg protein per electrophoresis zone.

Key concepts: Coomassie Brilliant Blue, Staining, Chemistry, Hydrochloric acid, Reagent, Chromatography, Electrophoresis, Molecular biology

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