The Effects of bFGF on Proliferation and Apoptosis of Fibroblasts in Human Hypertrophic Scar

Objective:To investigate the effects of bFGF on proliferation cell cycles and apoptosis of fibroblasts in human hypertrophic scar.Methods:Primary fibroblasts were obtained by the explant culture of resected hypertrophic scar from 3 patients.The second passages were cultured in DMEM supplemented with 1,5,10,50,100,500 ng/mL of bFGF respectively for 72 hours.The control was cultured without bFGF.MTT was used to detect the proliferation of scar-derived fibroblasts. The cell cycle and the percentage of apoptosis cells were measured by flow cytometry.Results: ①The value of OA was increased from 0.1833±0.019 to 0.3432±0.041(P0.05) when the concentration of bFGF added up,and the effect was dose dependent.②bFGF could interfere with the cellular cycle.While bFGF concentration increased,the percent age of cells in G1 phase decreased from 81.3%±3.24% to 75.9%±1.21%,and the percent age of apoptosis cells decreased from 7.2%±0.56% to 2.3%±0.49%(P0.05).Conclusion: bFGF could promote the proliferation of fibroblasts in human hypertrophic scar by stimulating mitosis,as well as protect it from apoptosis.