The factors influencing on the induction of callus in Amor phophallus

Effects of several factors such as different explants,hormones,mediums and physiological periods of explants on the induction of callus in Amor phophallus and differentiation ability of callus from different treatments were studied. Results showed that the apical blossom bud was the best explants for inducing to form callus,next was the infancy bract,the daughter corms and root corms could also be induced but they had low induced rate. The daughter corms and the root corms treated by low temperature at 4 ℃ had better induced effect than the fresh explants. Four explants were all inducted to form callus in media such as MS+NAA1.0～2.0 mg/L+BA1.0～2.0 mg/L,the induced rate was mostly above 50% and the highest ratio was 87.5%. Results also showed that the explants gathering at growth period were more easy to be induced to form callus than that of gathering at dormancy period. There was distinct difference between callus induced from different explants and induced medium.