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Effect of Rb_1 on adult rat cardiomyocytes apoptosis

Wen Zhang

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Abstract

Objective:To examine whether the apoptosis of adult cardiomyocytes could be induced by ischemia reperfusion,and to observe the effect of Rb 1 on the apoptosis.Methods:Agarose gel electrophoresis,TdT mediated dUTP nick end labeling (TUNEL) and fluorescence spectrophotometry were used.Results:The group of ischemia reperfusion showed characteristic DNA ladders in agarose gels;the percentage of positive cells in TUNEL and the DNA fragmentation observed with fluorospectrophotometer were higher than that of control.The group of Rb 1 showed that the percentage of apoptosis in TUNEL was obviously reduced,and the percentage of DNA freagmentation was also less than that of control.Conclusion:The apoptosis of adult cardiomyocytes could be induced by ischemia reperfusion injury and inhibited by Rb 1.

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What this paper is about

Objective:To examine whether the apoptosis of adult cardiomyocytes could be induced by ischemia reperfusion,and to observe the effect of Rb 1 on the apoptosis.Methods:Agarose gel electrophoresis,TdT mediated dUTP nick end labeling (TUNEL) and fluorescence spectrophotometry were used.Results:The group of ischemia reperfusion showed characteristic DNA ladders in agarose gels;the percentage of positive cells in TUNEL and the DNA fragmentation observed with fluorospectrophotometer were higher than that of control.The group of Rb 1 showed that the percentage of apoptosis in TUNEL was obviously reduced,and the percentage of DNA freagmentation was also less than that of control.Conclusion:The apoptosis of adult cardiomyocytes could be induced by ischemia reperfusion injury and inhibited by Rb 1.

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Available abstract

Objective:To examine whether the apoptosis of adult cardiomyocytes could be induced by ischemia reperfusion,and to observe the effect of Rb 1 on the apoptosis.Methods:Agarose gel electrophoresis,TdT mediated dUTP nick end labeling (TUNEL) and fluorescence spectrophotometry were used.Results:The group of ischemia reperfusion showed characteristic DNA ladders in agarose gels;the percentage of positive cells in TUNEL and the DNA fragmentation observed with fluorospectrophotometer were higher than that of control.The group of Rb 1 showed that the percentage of apoptosis in TUNEL was obviously reduced,and the percentage of DNA freagmentation was also less than that of control.Conclusion:The apoptosis of adult cardiomyocytes could be induced by ischemia reperfusion injury and inhibited by Rb 1.

Key concepts: TUNEL assay, Apoptosis, Agarose gel electrophoresis, DNA fragmentation, Agarose, In Situ Nick-End Labeling, Molecular biology, Ischemia

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