Effect of Ca~(2+) on Activities of Some Enzymes in Tobacco Seedlings under Cold Stress
Zhang Yan Fang LiLI Tian-Fei Yao Zhao-BingFENG Yong-XinWU Ye-Chi
Abstract
Zhang Yan Fang LiLI Tian-Fei Yao Zhao-BingFENG Yong-XinWU Ye-Chi
Abstract
After submerging tobacco seeds with CaCl 2 solution, we studied the effect of Ca 2+ on activities of certain enzymes in tobacco seedlings. It was shown that submerging tobacco seeds with CaCl 2 solution increased the content of combinative calcium in tobacco seedlings and the protective enzyme activity, and reduced the permeability of cell membrane and the content of MDA. Under the low temperature stress, the reduction of SOD, POD and CAT activities in the tobacco seedlings treated with Ca 2+ was lower than that of control seedlings . The relative electric conductivity of cells in tobacco seedling treated with Ca 2+ also reduced. The recovery of cell membrane permeability of tobacco seedlings treated with Ca 2+ was faster than that of control seedlings after seedling growth resumed. CPZ, the special inhibitor of CaM, could partly restrain the function of Ca 2+ in increasing the activities of SOD, CAT and POD.
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After submerging tobacco seeds with CaCl 2 solution, we studied the effect of Ca 2+ on activities of certain enzymes in tobacco seedlings. It was shown that submerging tobacco seeds with CaCl 2 solution increased the content of combinative calcium in tobacco seedlings and the protective enzyme activity, and reduced the permeability of cell membrane and the content of MDA. Under the low temperature stress, the reduction of SOD, POD and CAT activities in the tobacco seedlings treated with Ca 2+ was lower than that of control seedlings . The relative electric conductivity of cells in tobacco seedling treated with Ca 2+ also reduced. The recovery of cell membrane permeability of tobacco seedlings treated with Ca 2+ was faster than that of control seedlings after seedling growth resumed. CPZ, the special inhibitor of CaM, could partly restrain the function of Ca 2+ in increasing the activities of SOD, CAT and POD.
Key concepts: Seedling, Point of delivery, Membrane permeability, Enzyme, Calcium, Chemistry, Enzyme assay, Horticulture