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Using Antibodies Against Taq-FS Polymerase for Hot-start PCR

Lin Qing

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Abstract

Hot-start PCR is advantageous for its higher speciality,sencitivity and linear amplification.We have used an antibodies against Taq-FS polymerase for the setup hot-start PCR reactions.The antibody used could block the activity of Taq-FS polymerase at low tempreture(or below).When heated at high temperature 95℃,1 min,the antibody was denatured and the activity of Taq-FS polymerase was released.Our hot-start PCR system was capable of detecting as low as one copy of template DNA.

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What this paper is about

Hot-start PCR is advantageous for its higher speciality,sencitivity and linear amplification.We have used an antibodies against Taq-FS polymerase for the setup hot-start PCR reactions.The antibody used could block the activity of Taq-FS polymerase at low tempreture(or below).When heated at high temperature 95℃,1 min,the antibody was denatured and the activity of Taq-FS polymerase was released.Our hot-start PCR system was capable of detecting as low as one copy of template DNA.

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Available abstract

Hot-start PCR is advantageous for its higher speciality,sencitivity and linear amplification.We have used an antibodies against Taq-FS polymerase for the setup hot-start PCR reactions.The antibody used could block the activity of Taq-FS polymerase at low tempreture(or below).When heated at high temperature 95℃,1 min,the antibody was denatured and the activity of Taq-FS polymerase was released.Our hot-start PCR system was capable of detecting as low as one copy of template DNA.

Key concepts: Hot start PCR, Taq polymerase, Molecular biology, Polymerase chain reaction, Polymerase, Chemistry, DNA polymerase, Multiple displacement amplification

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