Expresssion of NUMBL after experimental spinal cord injury

[Objective]To observe the expression patterns of NUMBL in rat models of spinal cord injury,and analysis the association with neuronal survival or apoptosis and glial proliferation,as well as to investigate their potential biological roles in nerve injury and regeneration.[Method]Sprague-Dawley(SD) rats were subjected to spinal cord injury.In the purpose of exploring the pathological and physiological significance of NUMBL,the expression patterns and distribution for NUMBL were observed by western blot,immunohistochemistry and immunofluorescence.[Result]Western blot analysis showed NUMBL protein level was significant up-regulated at 8 hours and 1 day,then reduced and had no up-regulation to 2 weeks.Immunohistochemistry analysis showed that the immunochemical staining was significantly increased at 1 day and then reduced at 1 week.Immunoflurescence analysis showed NUMBL were expressed in neurons and oligodendrocytes but not in astrocytes.[Conclusion]The up-regulation of NUMBL in the injured spinal cord may be associated with apoptosis process of neurons and oligodendrocytes.