Approaches To Isolate,Culture and Identify The Rabbit Marrow Mesenchymal Stem Cells

Objective:To explore the approaches to isolate,culture and identify the bone marrow mesenchymal stem cells(MSCs) of rabbits in vitro.Methods:Rabbit MSCs were extracted through the density gradient centrifugation and separation of the combination adherent, cell growth rate and morphological changes was observed using the inverted microscope,select the P3 cells induced using special media supplemented,observe the feasibility of differentiation into bone cells,fat cells,cartilage cells.Expression of CD29,CD44,CD34,CD45 was detected using flow cytometry.Results:The cultured cells showing long fusiform and fibroblast-like growth,plastic attachment and rapid growth of passage cell,they can differentiate into mature bone cells,cartilage cells and fat cells under induction.Flow cytometry results showed that for the P3 cells,about 92.5%of the cells expressed CD29,about 86%of the cells expressed CD44.0.77%of the cells expressed CD34,0.32%of the cells expressed CD45.Conclusion:The density gradient separation and screening adherence combination isolated from rabbit bone marrow in vitro with high purity MSCs,vigorous growth,proliferation ability,under the conditions with the induction of osteoblasts,adipose cells and cartilage cells potential.