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2016•Journal of Entomology and Zoology StudiesOpen access

Molecular detection of cutaneous leishmaniasis in human at district Hangu Khyber Pakhtunkhwa, Pakistan

A. Rehman, Hameed Ur Rehman, Sultan Ayaz, Haider Gul, Muhammad Tahseen, Muhammad Ishaq, Sami Ullah, Sajid Ullah Shakir, Samina Jehan

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Abstract

Cutaneous Leishmaniasis is parasitic disease which causes severe morbidity and mortality. The present study identified the leishmania infection caused by Leishmania tropica in Hangu. A total of 45 samples were examined through PCR, Microscopy which showed 87.61% (99/113), 53.98% (61/113) thus the sensitivity of PCR is very high as compared to microscopy. During the study 186 bp Leishmania tropica was identified through PCR and presence of Leishmania tropica showed the infected person serum through SDS method analysis respectively.

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What this paper is about

Cutaneous Leishmaniasis is parasitic disease which causes severe morbidity and mortality. The present study identified the leishmania infection caused by Leishmania tropica in Hangu. A total of 45 samples were examined through PCR, Microscopy which showed 87.61% (99/113), 53.98% (61/113) thus the sensitivity of PCR is very high as compared to microscopy. During the study 186 bp Leishmania tropica was identified through PCR and presence of Leishmania tropica showed the infected person serum through SDS method analysis respectively.

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Available abstract

Cutaneous Leishmaniasis is parasitic disease which causes severe morbidity and mortality. The present study identified the leishmania infection caused by Leishmania tropica in Hangu. A total of 45 samples were examined through PCR, Microscopy which showed 87.61% (99/113), 53.98% (61/113) thus the sensitivity of PCR is very high as compared to microscopy. During the study 186 bp Leishmania tropica was identified through PCR and presence of Leishmania tropica showed the infected person serum through SDS method analysis respectively.

Key concepts: Leishmania tropica, Cutaneous leishmaniasis, Khyber pakhtunkhwa, Leishmania, Leishmaniasis, Leishmania major, Parasitic disease, Biology

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