Preparation and identification of anti-EPF monoclonal antibodies

Objective To establish hybridoma cell lines secreting anti-early pregnancy factor (EPF) antibodies, and to select monoclonal antibodies of high affinity and specificity from the cell lines. Methods Spleen cells from BALB/C mice immunized with purified EPF were fused with murine NS-1 myeloma cells. Ascite fluid containing the monoclonal antibodies against EPF was obtained by ventral injection. Monoclonal antibodies were purified by protein-A affinity chromatography. Results One hybridoma cell line (C3D11) of anti-EPF was obtained, which could stably secrete the monoclonal antibodies. Ascites containing the antibodies was obtained through subcloning the obtained cell lines for4 cycles. With a high purity, the obtained antibodies had and could match the specific antigens perfectly. Conclusion The monoclonal antibodies are aimed at EPF.