The Polymorphism of HLA-B Alleles in Sichuan Yi and Xinjiang Uygur Ethnic Groups

The polymorphism of HL A-B alleles in Sichuan Yi and Xinjiang Uygur population were investigated using the PCR-SSP method.Twenty alleles were detected in HLA-B loci in 106 Sichuan unrelated Yi healthy subjects.Of them,B40, B15 and B51 were the most common alleles with an allele frequency of 0.2208,0.1604,0.1274 respectively;B47,B27,B44,B18 and B78 were the rare alleles with an allele frequency of 0.0189,0.0142,0.0142,0.0094 and 0.0047 respectively.In 110 Xinjiang unrelated healthy Uygur subjects,27 alleles were detected in HLA-B loci.Of them,B35 and B51 were the most common alleles with an allele frequency of 0.1136 and 0.1136 respectively;B41,B56 and B78 were the rare alleles with a frequency of 0.0045,0.0045 and 0.0091 respectively.x~2 tests showed that HLA-B loci of the two ethnic groups both accorded with Hardy-Weinberg equilibrium.Heterozygosity(H),discrimination power(DP)and probability of paternity exclusion(EP)of HLA-B locus from Sichuan Yi ethnic group were computed to be 0.8977,0.9661 and 0.8009;and those from Xinjiang Uygur ethnic group as 0.9372,0.9857 and 0.8732.This study obtained data on the distribution of HL A-B alleles in the Sichuan Yi and Xinjiang Uygur population and the data can be used in forensic and paternity tests to estimate the frequency of a DNA profile in these two populations,transplant matching,anthropology and disease association study.