Expression of Survivin Induced by Dially Disulfide in HepG2 Cells

Objective:To investigate the expression of survivin induced by diallyl disulfide (DADS),and determine the role in chemotherapy of hepatocellular carcinoma (HepG2).Methods:The growth inhibition of HepG2 cells was measured by MTT assay.The Phase distribution of cell cycle and apoptosis was analyzed by flow cytometry.The expression of survivin was detected by RT-PGR and western-blot.Results:After treatment with 25-200μmol/l DADS for 24 h,MTT assay showed that HepG2 cell viability,compared with the control group,dropped to 9.3%,10.4%,21.6% and 31.2%,respectively.Flow cytometry analysis showed that apoptosis rate,compared with the control group,increased 0.83%,1.97%,6.0% and 9.9% respectively.RT- PGR assay revealed that DADS of low concentration (25,50μmol/l) could induce the increasing expression of survivin mRNA, while the DADS of high concentration (100,200μmol/l) was opposite.Western blot analysis showed the protein of survivin was decreased with the concentration increase.Conclusion:The expression of survivin was induced in HepG2 by DADS and resisted to DADS-mediated apoptosis.