Immunoscreening of cDNA Expression Library of Haemaphysalis qinghaiensis and Characterization of Positive Clones

To obtain the immunogen against Haemaphysalis qinghaiensis,a cDNA expression library of H.qinghaiensis was screened with rabbit anti-H.qinghaiensis differential protein serum and rabbit anti-H.qinghaiensis saliva protein serum.Totally 58 positive signals were obtained.Pure phage stocks were harvested by plaque purification and converted to plasmid subclones by plating phage on host strain BM25.8.Recombinant plasmids that were subcloned in BM25.8 were isolated and then transformed into host strain JM109.Twenty one positive new clones were obtained after analysis of plasmids abstracted from JM109 by PCR,restriction digestion and sequencing.Five of the new clones were submitted to the GenBank/DDBJ/EMEL nucleotide sequence database.