2011•Unpublished venueRequires access
Separation and Purification of Three Xanthones from Swertia Mussotti Franch by High Speed Counter-Current Chromatography
Jia Jing, Yulin Li, Xiaohui Zhao, Yuancan Xiao, Guichen Chen, Jinmao You, Lixin Wei
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Abstract
High speed counter-current chromatographic (HSCCC) method was used to purify and separate methylswertianin, gentiacaulein and decussatin from Swertia mussotti Franch. with a two-phase solvent system composed of hexane-ethyl acetate-ethanol-water (5 : 5 : 6 : 4, V/V). The upper phase was used as the stationary phase and the lower phase was used as the mobile phase at a flow rate of 2.5 mL/min. The experimental conditions were controlled at 850 r/min and 25 degrees C and the effluent was detected at 254 nm. The separation producs of methylswertianin, gentiacaulein and decussatin with the purity of over 98% were obtained by high performance liquid chromatography, and identification of their structures was performed by (1)H NMR, (13)C NMR and 2D NMR.