Evaluation of quantitative measurement of large immature cell and atypical lymphocyte in peripheral blood using BC-5500 hematology analyzer

Objective To evaluate the performance of measurement of large immature cell(LIC) and atypical lymphocyte(ALY) in peripheral blood using BC-5500 hematology analyzer.Methods Each 200 positive samples with LIC and ALY alarm value3% were selected to compare BC-5500 count and manual morphology count;100 negative samples without alarm were selected to compare BC-5500 and the manual morphology count.5 samples at different levels were selected to evaluate within-run reproducibility for BC-5500 LIC% and ALY% count,8 specimens were selected to evaluate the sample stability at 4℃ and at room temperature.Results In 200 positive samples,the comparison analysis showed LIC% coincidence rate was 26.5% between BC-5500 count and manual morphology count.In 200 positive samples the comparison analysis showed ALY% coincidence rate was between BC-5500 count and manual morphology count,the falsepositive rates was 73.5% and 30.5%.In 100 negative samples without alarm,comparison analysis showed LIC% and ALY% coincidence rate was 96% and 68% between BC-5500 count and manual morphology.The within-run reproducibility performed using fresh blood on 5 different LIC% level specimens showed a range from 3.22% to 6.25% CV and the ALY% specimens showed a range from 3.25% to 6.12% CV.The percent of LIC% and ALY% were stable at both room temperature and after refrigeration for 24 h.Conclusion In the performance of quantitative measurement of LIC and ALY in peripheral blood using BC-5500 hematology,accuracy is poor,showing a high false positive and false-negative,the instrument alarm function for immature cells still needs to be improved for meeting the requirements of clinical laboratories.