Pollen mother cell mitosis and male gametophyte development of Raphanus sativus L.

Chromosomal meiotic behaviors and male gametophytes development of Raphanus sativus L.were studied using chromosome preparation and Ehrlich′s hematoxylin staining-methyl salicylate clearing technique.The results were as follows:the meiosis in pollen mother cells of radish simultaneous cytokinesis and chromosomes could be identified as rod or ring bivalents in diakinesis except for 3% abnormal.A few lagging chromosomes appeared in metaphases Ⅰand the spindle arranged in vertical,parallel and the'八'like in metaphase Ⅱ.The microspores in tetrad stage showed the arrangement of decussate type,tetrahedral type and isobilateral type.The mature pollen grains contained three apertures and three nucleus which were made up of two pronucleus and one alimentation nucleus.The buds larged from 2.0 to 4.5 mm were usually at uninucleate stage which were adapt for microspore culture.About 87.9% of pollens were activated with the rest aborted at uninucleate stage.