2010•Zhongguo fuyou baojianRequires access
The expression of LYVE-1 in cervical squamous cell carcinoma and the relationship between tumor lymphangiogenesis and cervical squamous cell carcinoma metastasis through the lymphatic system
Zhang Yi
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Abstract
Objective:To investigate the distribution patterns and proliferative activity of lymphatic vessels in cervical squamous cell carcinoma (CSCC) through double-labeling immunohistochemistry with antibody to LYVE-1 as a marker of lymphatic vessel-specific marker, explore the rule of tumor lymphatic genesis and CSCC metastasis through the lymphatic system.Methods:80 cases of CSCC and the corresponding normal tissue were obtained, microlymphatic density(MLD) was evaluated by immunohistochemistry with LYVE-1 antibodies respectively.The Ki-67 expression at lymphatic was detected by double-labeling immunohistochemistry.Results:The lymphatic vessel at the tumor border of CSCC had large and open lumina, while those at other portions of CSCC often had a reticular architecture with numerous tiny and ill-defined lumina. The MLD in CSCC was notable higher than that in the normal tissue; the MLD were notable higher at the tumor border of CSCC, the group of low differentiation degree and the group with lymph node metastasis, there was no distinct difference of the number of MLD between stage Ⅰand Ⅱ CSCC. The Ki-67 index of lymphatic vessels endothelial in CSCC was notable higher than that in the normal tissue.The Ki-67 index of the edge area of CSCC was higher than other areas(P0.05), the group with lymph node metastasis was significantly higher.Conclusion:There are newly born lymphatic vessels in CSCC. The most lymphatic vessels live at tumor border of CSCC and the proliferative activity of lymphatic endothelial cells is increased. There is no definite correlation between clinical FIGO stage of CSCC with the MLD of CSCC, while the MLD of CSCC is related to the differentiation degree of CSCC and the with lymph node metastasis. The detection of MLD and proliferative activity of lymphatic vessels in CSCC may be valuable in predicting lymphatic metastasis.