The effects of sulforaphane on the proliferation and apoptosis of salivary adenoid cystic carcinoma ACC-M cells

Objective: To study the effects of sulforaphane( SFN) on the proliferation and apoptosis of salivary adenoid cystic carcinoma ACC-M cells in vitro. Methods: ACC-M cells were treated with SFN at the doses( μmol /L) of 5,10,20,30,40,60,80 and 100 for 24,48 and 72 hours,respectively. The growth inhibition was examined with MTT assay and typan blue exclusion assay. Morphology of ACC-M cells was observed with phase contrast microscope,giemsa staining and transmission electron microscope. Flow cytometry with Annexin-V-FITC /PI double staining was used to detect the apoptosis of ACC-M cells. Results: SFN inhibited the proliferation of ACC-M cells,the IC50values( μmol /L) after 24,48 and 72 h treatment were 75. 6,21. 3 and 16. 5 respectively. The highest inhibition rate was 89. 2%. The growth inhibition rate of SFN on ACC-M cells was positively correlated with concentrations of SFN and treatment time. SFN induced the apoptosis of ACC-M cells in a dose and time dependent manner( P 0. 01). Conclusion: SFN can inhibit proliferation and induce apoptosis of salivary adenoid cystic carcinoma ACC-M cells time and dose-dependently.